Publications Repository - Helmholtz-Zentrum Dresden-Rossendorf

Heterologous expression is a common method of molecular biology, which finds applications for example in up scaling of protein production and structural analyses of naturally unexpressed proteins. An experimental setup is started by the amplification of the template gene. Afterwards this gene is cloned into coiled DNA, called vectors and transformed into the host organism. The desired protein, which is encoded by the cloned gene, is produced inside the host organism. Host strains are for example Escherichia coli, Lactococcus lactis or Pichia pastoris.