Publications Repository - Helmholtz-Zentrum Dresden-Rossendorf
1 PublicationHierarchical thermoplastic rippled nanostructures regulate Schwann Cell adhesion, morphology and spatial organization
Masciullo, C.; Dell'Anna, R.; Tonazzini, I.; Böttger, R.; Pepponi, G.; Cecchini, M.
Abstract
Periodic ripples are a variety of anisotropic nanostructures that can be realized by ion beam irradiation on a wide class of solid surfaces. Only few authors have investigated these surfaces for tuning the response of biological systems, probably because it is challenging to directly produce them in materials that well sustain long-term cellular cultures. Here, hierarchical rippled nanotopographies with lateral periodicity of ∽300 nm are produced from a gold-irradiated germanium mold in polyethylene terephthalate (PET), a biocompatible polymer approved by the US Food and Drug Administration for clinical applications, by a novel three-steps embossing process. The effects of nano-ripples on Schwann Cells (SCs) are studied in view of their possible use for nerve-repair applications. Data demonstrate that nano-ripples can enhance short-term SC adhesion and proliferation (3-24h from seeding), drive their actin cytoskeleton spatial organization and sustain long-term cell growth. Notably, SCs orient perpendicularly with respect to the nanopattern lines. These results provide information about the possible use of hierarchical nano-rippled elements for nerve-regeneration protocols.
Keywords: hierarchical nanostructures; self-organization; ion Irradiation; cell adhesion; nano-ripples
Involved research facilities
- Ion Beam Center DOI: 10.17815/jlsrf-3-159
Related publications
- DOI: 10.17815/jlsrf-3-159 is cited by this (Id 26037) publication
-
Nanoscale 9(2017)39, 14861-14874
DOI: 10.1039/C7NR02822A
Cited 19 times in Scopus
Permalink: https://www.hzdr.de/publications/Publ-26037
Years: 2023 2022 2021 2020 2019 2018 2017 2016 2015